Hotspot

rpl32-trnL-UAG

SSC · 110209-111132 · 924 bp

Back to Elaeagnus

Pi 0.0295

MarkerSeek score 66.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 924
variable_sites: 84
pi: 0.0295
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCATTGAAAGCCTTTT	ATAACACTTGGCGCGGTTAT	45.0	45.0	57.8	58.0	1416-1569 bp	1.0	88.9
rpl32-trnL-UAG_p2	GGCAGCATTGAAAGCCTTTT	AAAAATAACACTTGGCGCGG	45.0	45.0	57.8	57.9	1420-1573 bp	1.0	88.7
rpl32-trnL-UAG_p3	AGCATATTGGGCAGCATTGA	ATAACACTTGGCGCGGTTAT	45.0	45.0	57.9	58.0	1425-1527 bp	0.916667	84.6
rpl32-trnL-UAG_p4	AGCATATTGGGCAGCATTGA	AAAAATAACACTTGGCGCGG	45.0	45.0	57.9	57.9	1429-1531 bp	0.916667	84.4
rpl32-trnL-UAG_p5	AGCATATTGGGCAGCATTGA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	943-1051 bp	0.916667	84.2