Hotspot

ndhF-rpl32

SSC · 108818-110052 · 1235 bp

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Pi 0.0193

MarkerSeek score 61.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1235
variable_sites: 77
pi: 0.0193
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAAGGAAAGGT	TTTTTGATATAGAGGTACGTTTTT	50.0	25.0	57.0	52.7	1213-1324 bp	1.0	49.0
ndhF-rpl32_p2	CTGGAAGTGGAAGGAAAGGTA	TTTTTGATATAGAGGTACGTTTTT	47.6	25.0	56.9	52.7	1213-1324 bp	1.0	49.0
ndhF-rpl32_p3	TGGAAGTGGAAGGAAAGGTATG	TTTTTGATATAGAGGTACGTTTTT	45.5	25.0	57.7	52.7	1212-1323 bp	1.0	49.0
ndhF-rpl32_p4	TGGAAGTGGAAGGAAAGGTA	TTTTTGATATAGAGGTACGTTTTT	45.0	25.0	55.7	52.7	1212-1323 bp	1.0	49.0
ndhF-rpl32_p5	GTGGAAGGAAAGGTATGATCCA	TTTTTGATATAGAGGTACGTTTTT	45.5	25.0	57.5	52.7	1207-1318 bp	1.0	49.0