Hotspot

rps8-rpl14

LSC · 77379-77534 · 156 bp

Back to Elaeagnus

Pi 0.0129

MarkerSeek score 64.40

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 8
pi: 0.0129
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GAGGGTACTCGAACTGTTCT	GGGAACTCGAATTTTTGGCG	50.0	50.0	56.3	58.0	327-371 bp	1.0	72.3
rps8-rpl14_p2	GAGGGTACTCGAACTGTTCTT	GGGAACTCGAATTTTTGGCG	47.6	50.0	57.1	58.0	327-371 bp	1.0	70.3
rps8-rpl14_p3	GAGGGTACTCGAACTGTTCT	CGCCCGGGAATTAAGACAAT	50.0	50.0	56.3	58.3	303-347 bp	1.0	70.0
rps8-rpl14_p4	GAGGGTACTCGAACTGTTCT	GATCGCCCGGGAATTAAGAC	50.0	55.0	56.3	58.5	306-350 bp	1.0	68.7
rps8-rpl14_p5	AGGGTACTCGAACTGTTCTT	GGGAACTCGAATTTTTGGCG	45.0	50.0	55.8	58.0	326-370 bp	1.0	68.3