Hotspot

psaJ-rpl33

LSC · 64259-64658 · 400 bp

Back to Elaeagnus

Pi 0.0222

MarkerSeek score 61.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 400
variable_sites: 36
pi: 0.0222
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CGGCTTTGGCAGGTTTATTG	GGCATCTTTACCTTTGGCCA	50.0	50.0	58.0	58.4	473-492 bp	1.0	86.7
psaJ-rpl33_p2	CGGCTTTGGCAGGTTTATTG	TCGGGCATCTTTACCTTTGG	50.0	50.0	58.0	57.9	495 bp	0.083333	42.3
psaJ-rpl33_p3	TCGTTTATTTCCGGATGCGT	TCGGGCATCTTTACCTTTGG	45.0	50.0	58.0	57.9	464 bp	0.083333	42.2
psaJ-rpl33_p4	TCCGGATGCGTTGATATTCC	TCGGGCATCTTTACCTTTGG	50.0	50.0	57.8	57.9	455 bp	0.083333	41.0
psaJ-rpl33_p5	CGGATGCGTTGATATTCCCT	TCGGGCATCTTTACCTTTGG	50.0	50.0	57.8	57.9	453 bp	0.083333	41.0