Hotspot

trnL-UAA-trnF-GAA

LSC · 46811-47191 · 381 bp

Back to Elaeagnus

Pi 0.0265

MarkerSeek score 49.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 381
variable_sites: 23
pi: 0.0265
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	375-471 bp	1.0	84.5
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	390-486 bp	1.0	84.0
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	CATGTGCCAGGAACCAGATT	50.0	50.0	57.5	58.2	393-489 bp	1.0	83.4
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	337-433 bp	1.0	81.8
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TGTGCCAGGAACCAGATTTG	50.0	50.0	57.5	58.4	391-487 bp	1.0	81.7