Hotspot

trnC-GCA-petN

LSC · 26603-27260 · 658 bp

Back to Elaeagnus

Pi 0.0157

MarkerSeek score 63.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 36
pi: 0.0157
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	728-908 bp	1.0	85.4
trnC-GCA-petN_p2	TTTTGTATTGTTTGGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.8	58.2	785-965 bp	1.0	84.8
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	789-969 bp	1.0	83.7
trnC-GCA-petN_p4	CCCCTTTCCCCAGTTCAAAT	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.7	58.2	732-912 bp	1.0	83.5
trnC-GCA-petN_p5	TTTTGTATTGTTTGGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.8	57.6	846-1026 bp	1.0	82.9