Hotspot

trnS-GCU-trnG-GCC

LSC · 7149-7654 · 506 bp

Back to Elaeagnus

Pi 0.0411

MarkerSeek score 83.10

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 506
variable_sites: 73
pi: 0.0411
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ATCAAACCGAGGACCCCTTA	50.0	50.0	58.0	58.0	601-709 bp	1.0	91.7
trnS-GCU-trnG-GCC_p2	CAATCCGACGCTTTAGTCCA	TAATCAAACCGAGGACCCCT	50.0	50.0	58.0	58.0	603-711 bp	1.0	91.7
trnS-GCU-trnG-GCC_p3	AATCCGACGCTTTAGTCCAC	ATCAAACCGAGGACCCCTTA	50.0	50.0	58.0	58.0	600-708 bp	1.0	91.6
trnS-GCU-trnG-GCC_p4	AATCCGACGCTTTAGTCCAC	TAATCAAACCGAGGACCCCT	50.0	50.0	58.0	58.0	602-710 bp	1.0	91.6
trnS-GCU-trnG-GCC_p5	CAATCCGACGCTTTAGTCCA	CAAACCGAGGACCCCTTAAC	50.0	55.0	58.0	58.2	599-707 bp	1.0	90.5