Hotspot

petD

LSC · 72468-73699 · 1232 bp

Back to Ehrharta

Pi 0.0171

MarkerSeek score 58.10

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1232
variable_sites: 21
pi: 0.0171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACTTCTCTTCAACTCCGAAGT	ACACGGTACTTCACGATTGA	42.9	45.0	56.6	56.9	1345-1348 bp	1.0	53.6
petD_p2	GCTACAAGCATGGGTTATTGT	ACACGGTACTTCACGATTGA	42.9	45.0	56.6	56.9	1389-1392 bp	1.0	53.2
petD_p3	ACTTCTCTTCAACTCCGAAGT	ACACGGTACTTCACGATTGAC	42.9	47.6	56.6	58.3	1345-1348 bp	1.0	51.7
petD_p4	GCTACAAGCATGGGTTATTGT	ACACGGTACTTCACGATTGAC	42.9	47.6	56.6	58.3	1389-1392 bp	1.0	51.3
petD_p5	ACTTCTCTTCAACTCCGAAGT	CACGGTACTTCACGATTGAC	42.9	50.0	56.6	56.6	1344-1347 bp	1.0	51.0