Hotspot

clpP

LSC · 67200-67850 · 651 bp

Back to Ehrharta

Pi 0.00768

MarkerSeek score 48.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 651
variable_sites: 5
pi: 0.00768
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGACCCTGTTAGTCCGTTCT	CCACCCCACGAATTGGTTAT	50.0	50.0	58.0	57.9	1916-1927 bp	1.0	80.0
clpP_p2	TGACCCTGTTAGTCCGTTCT	CCAGAACAGGATCAGAGGGA	50.0	55.0	58.0	58.1	1843 bp	0.5	58.9
clpP_p3	TGACCCTGTTAGTCCGTTCT	TCCAGAACAGGATCAGAGGG	50.0	55.0	58.0	58.1	1844 bp	0.5	58.9
clpP_p4	CTGAACGAGTCGCACATACA	CCAGAACAGGATCAGAGGGA	50.0	55.0	58.0	58.1	1584 bp	0.5	58.8
clpP_p5	CTGAACGAGTCGCACATACA	TCCAGAACAGGATCAGAGGG	50.0	55.0	58.0	58.1	1585 bp	0.5	58.8