Hotspot

rpl32-trnL-UAG

SSC · 104602-105184 · 583 bp

Back to Ehrharta

Pi 0.0275
MarkerSeek score 64.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
583
variable_sites
16
pi
0.0275
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TTTTCTACAATGCCGCCACT 45.0 45.0 58.1 58.1 718-724 bp 1.0 80.1
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TTTTCTACAATGCCGCCACT 45.0 45.0 58.1 58.1 717-723 bp 1.0 80.1
rpl32-trnL-UAG_p3 AAGGGTTTTTCTGGGCAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 667-673 bp 1.0 80.0
rpl32-trnL-UAG_p4 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 666-672 bp 1.0 80.0
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 659-665 bp 1.0 79.6