Hotspot

rps3-rpl22

LSC · 79615-79674 · 60 bp

Back to Ehrharta

Pi 0.0179

MarkerSeek score 66.60

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 1
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GTGCGAACCAAAAGGAATGG	AAAAATTCAGACCTCGGGCC	50.0	50.0	57.9	58.5	227-231 bp	1.0	77.6
rps3-rpl22_p2	GTTGTGCGAACCAAAAGGAA	AAAAATTCAGACCTCGGGCC	45.0	50.0	57.4	58.5	230-234 bp	1.0	73.5
rps3-rpl22_p3	TGTGCGAACCAAAAGGAATG	AAAAATTCAGACCTCGGGCC	45.0	50.0	57.2	58.5	228-232 bp	1.0	71.8
rps3-rpl22_p4	GTGCGAACCAAAAGGAATGG	AAAATTCAGACCTCGGGCC	50.0	52.6	57.9	57.7	226-230 bp	1.0	71.1
rps3-rpl22_p5	CCAAGTCTGAAACCAAGTGGA	AAAAATTCAGACCTCGGGCC	47.6	50.0	58.1	58.5	192-196 bp	1.0	69.9