Hotspot

psbF-psbE

LSC · 61636-61645 · 10 bp

Back to Ehrharta

Pi 0.1

MarkerSeek score 79.90

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 10
variable_sites: 1
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	TGGATGAACTGCATTGCTGA	GAAAGCCGACAAGGAATTCC	45.0	50.0	57.8	57.1	204 bp	1.0	71.4
psbF-psbE_p2	TGGATGAACTGCATTGCTGA	GGAAAGCCGACAAGGAATTC	45.0	50.0	57.8	57.1	205 bp	1.0	71.4
psbF-psbE_p3	AGGTACAGCTAGTCCGTGAA	GAAAGCCGACAAGGAATTCC	50.0	50.0	57.8	57.1	160 bp	1.0	71.4
psbF-psbE_p4	AGGTACAGCTAGTCCGTGAA	GGAAAGCCGACAAGGAATTC	50.0	50.0	57.8	57.1	161 bp	1.0	71.4
psbF-psbE_p5	GGTACAGCTAGTCCGTGAAC	GAAAGCCGACAAGGAATTCC	55.0	50.0	57.7	57.1	159 bp	1.0	70.9