Hotspot

trnL-UAA-trnF-GAA

LSC · 46823-47055 · 233 bp

Back to Ehrharta

Pi 0.0429

MarkerSeek score 69.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 10
pi: 0.0429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	GTTGTGAGGGTTCAAGTCCC	TTTGAACTGGTGACACGAGG	55.0	50.0	58.4	58.1	322-330 bp	1.0	78.9
trnL-UAA-trnF-GAA_p2	GTTGTGAGGGTTCAAGTCCC	CTGTGCCAGGAACCAGATTT	55.0	50.0	58.4	58.1	339-347 bp	1.0	78.5
trnL-UAA-trnF-GAA_p3	GTTGTGAGGGTTCAAGTCCC	GTGCCAGGAACCAGATTTGA	55.0	50.0	58.4	58.1	337-345 bp	1.0	78.5
trnL-UAA-trnF-GAA_p4	GTTGTGAGGGTTCAAGTCCC	TTTTTCTGTGCCAGGAACCA	55.0	45.0	58.4	57.8	344-352 bp	1.0	78.2
trnL-UAA-trnF-GAA_p5	AGTTGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.4	58.1	323-331 bp	1.0	76.9