Hotspot

atpI-atpH

LSC · 31584-32168 · 585 bp

Back to Ehrharta

Pi 0.0212

MarkerSeek score 73.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 585
variable_sites: 12
pi: 0.0212
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CGTTAGCCGCAGCCTATATC	AAGCAGCAGCAATTAGTGGA	55.0	45.0	58.4	57.8	643-657 bp	1.0	79.3
atpI-atpH_p2	GTTAGCCGCAGCCTATATCG	AAGCAGCAGCAATTAGTGGA	55.0	45.0	58.4	57.8	642-656 bp	1.0	79.3
atpI-atpH_p3	CGTTAGCCGCAGCCTATATC	GCAGTACCTTGACCAACTCC	55.0	55.0	58.4	58.2	710-724 bp	1.0	79.1
atpI-atpH_p4	GTTAGCCGCAGCCTATATCG	GCAGTACCTTGACCAACTCC	55.0	55.0	58.4	58.2	709-723 bp	1.0	79.1
atpI-atpH_p5	CGCAGCCTATATCGGTGAAT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.6	58.2	703-717 bp	1.0	79.1