Hotspot

rps16-trnQ-UUG

LSC · 5580-6389 · 810 bp

Back to Ehrharta

Pi 0.0161

MarkerSeek score 68.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 13
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	884-914 bp	1.0	79.9
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	895-925 bp	1.0	79.3
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	AGTGTCGCATAAAACCCGAA	45.0	45.0	58.1	57.8	953-983 bp	1.0	79.0
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	888-918 bp	1.0	78.8
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	AGTGTCGCATAAAACCCGAA	50.0	45.0	58.2	57.8	964-994 bp	1.0	78.5