Hotspot

ndhF

SSC · 112530-114755 · 2226 bp

Back to Ehretia

Pi 0.00779

MarkerSeek score 47.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 26
pi: 0.00779
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	CTGGTCAAACTCGGGGAAAA	50.0	50.0	58.0	58.0	2874-2901 bp	1.0	80.5
ndhF_p2	ACAGGAACAAGAGGGATCCA	CTGGTCAAACTCGGGGAAAA	50.0	50.0	58.0	58.0	2750-2777 bp	1.0	80.5
ndhF_p3	AAGAGGGATCCACCGAAGAA	CTGGTCAAACTCGGGGAAAA	50.0	50.0	58.0	58.0	2742-2769 bp	1.0	80.5
ndhF_p4	GCTTCCGAAATGAAGGGGAT	CTGGTCAAACTCGGGGAAAA	50.0	50.0	57.9	58.0	2773-2800 bp	1.0	80.4
ndhF_p5	AGGGTGGAAAGTGAGGAAGA	CTGGTCAAACTCGGGGAAAA	50.0	50.0	57.9	58.0	2815-2842 bp	1.0	79.9