Hotspot

trnL-CAA-ycf2

IRa · 146691-147407 · 717 bp

Back to Ehretia

Pi 0.00559

MarkerSeek score 61.50

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 717
variable_sites: 6
pi: 0.00559
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	AAAGAGATGGCTTTTCCCGG	50.0	50.0	58.0	58.2	832-837 bp	1.0	79.4
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	AAAGAGATGGCTTTTCCCGG	50.0	50.0	58.0	58.2	834-839 bp	1.0	79.4
trnL-CAA-ycf2_p3	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	826-831 bp	1.0	78.9
trnL-CAA-ycf2_p4	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	825-830 bp	1.0	78.9
trnL-CAA-ycf2_p5	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	827-832 bp	1.0	78.9