Hotspot

ycf2-trnL-CAA

IRb · 95785-96501 · 717 bp

Back to Ehretia

Pi 0.00559

MarkerSeek score 61.50

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 717
variable_sites: 6
pi: 0.00559
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	AAAGAGATGGCTTTTCCCGG	AAATGGTAGACACGCGAGAC	50.0	50.0	58.2	58.0	832-837 bp	1.0	79.4
ycf2-trnL-CAA_p2	AAAGAGATGGCTTTTCCCGG	TGAAATGGTAGACACGCGAG	50.0	50.0	58.2	58.0	834-839 bp	1.0	79.4
ycf2-trnL-CAA_p3	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	826-831 bp	1.0	78.9
ycf2-trnL-CAA_p4	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	825-830 bp	1.0	78.9
ycf2-trnL-CAA_p5	TGGCTTTTCCCGGATGAAAT	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	827-832 bp	1.0	78.9