Hotspot

rpoA-rps11

LSC · 81471-81544 · 74 bp

Back to Ehretia

Pi 0.018

MarkerSeek score 63.00

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 74
variable_sites: 2
pi: 0.018
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	ACTTCCACTGTAGTGTCCGA	CACAATGGCTGTAGACCTCC	50.0	55.0	58.0	58.0	162 bp	1.0	80.7
rpoA-rps11_p2	TTCCACTGTAGTGTCCGAGT	CACAATGGCTGTAGACCTCC	50.0	55.0	58.0	58.0	160 bp	1.0	80.7
rpoA-rps11_p3	CACTTCCACTGTAGTGTCCG	CACAATGGCTGTAGACCTCC	55.0	55.0	57.9	58.0	163 bp	1.0	80.2
rpoA-rps11_p4	ACTTCCACTGTAGTGTCCGA	AAGAGACGCAGCATTACGAG	50.0	50.0	58.0	58.1	238 bp	1.0	79.9
rpoA-rps11_p5	TTCCACTGTAGTGTCCGAGT	AAGAGACGCAGCATTACGAG	50.0	50.0	58.0	58.1	236 bp	1.0	79.9