Hotspot

petA-psbJ

LSC · 65478-66477 · 1000 bp

Back to Ehretia

Pi 0.0114
MarkerSeek score 52.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1000
variable_sites
17
pi
0.0114
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CCCGGATCCTGCTACTAAGA GTCTCTAGCACGACCACTTG 55.0 55.0 58.0 58.0 1632-1662 bp 1.0 81.1
petA-psbJ_p2 CCCGGATCCTGCTACTAAGA AACTGGCCGTTTTGATCCTT 55.0 45.0 58.0 58.0 2043-2081 bp 1.0 80.9
petA-psbJ_p3 CCCGGATCCTGCTACTAAGA ACTGGCCGTTTTGATCCTTT 55.0 45.0 58.0 58.0 2042-2080 bp 1.0 80.9
petA-psbJ_p4 TCAGAGCTATCGCCCCAATA AACTGGCCGTTTTGATCCTT 50.0 45.0 58.0 58.0 2133-2171 bp 1.0 80.9
petA-psbJ_p5 TCAGAGCTATCGCCCCAATA ACTGGCCGTTTTGATCCTTT 50.0 45.0 58.0 58.0 2132-2170 bp 1.0 80.9