Hotspot

psaA-ycf3

LSC · 43460-44204 · 745 bp

Back to Ehretia

Pi 0.00649

MarkerSeek score 66.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 7
pi: 0.00649
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	893-905 bp	1.0	81.7
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	911-923 bp	1.0	81.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	840-852 bp	1.0	80.9
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	913-925 bp	1.0	80.8
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	844-856 bp	1.0	80.7