Hotspot

trnK-UUU-rps16

LSC · 4201-5105 · 905 bp

Back to Ehretia

Pi 0.0148
MarkerSeek score 58.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
905
variable_sites
19
pi
0.0148
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG TCGCCCTAATCAAACGGAAT 50.0 45.0 58.0 57.3 1011-1032 bp 1.0 77.9
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC TCGCCCTAATCAAACGGAAT 55.0 45.0 57.9 57.3 1028-1049 bp 1.0 77.5
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC TCGCCCTAATCAAACGGAAT 55.0 45.0 57.9 57.3 1027-1048 bp 1.0 77.5
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT TCGCCCTAATCAAACGGAAT 50.0 45.0 58.2 57.3 967-988 bp 1.0 77.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TCGCCCTAATCAAACGGAAT 50.0 45.0 58.2 57.3 966-987 bp 1.0 77.0