Hotspot

rps16

LSC · 5119-6302 · 1184 bp

Back to Edgeworthia

Pi 0.0121
MarkerSeek score 54.10
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1184
variable_sites
21
pi
0.0121
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 GTTGAGTTAGCAACCCCCAA ACTGCGTACTAAATGAACCGA 50.0 42.9 58.0 57.4 1973-1990 bp 0.666667 55.3
rps16_p2 TGAGTCATCCACACCCTCTT ACTGCGTACTAAATGAACCGA 50.0 42.9 58.0 57.4 1346-1350 bp 0.666667 55.0
rps16_p3 GCATTCGTTCAGATCCGCTA ACTGCGTACTAAATGAACCGA 50.0 42.9 58.1 57.4 1839-1856 bp 0.666667 54.1
rps16_p4 AAAGCCGAGTACTCTACCGT ACTGCGTACTAAATGAACCGA 50.0 42.9 58.2 57.4 1991-2008 bp 0.666667 54.0
rps16_p5 AAGCCGAGTACTCTACCGTT ACTGCGTACTAAATGAACCGA 50.0 42.9 58.2 57.4 1990-2007 bp 0.666667 54.0