Hotspot

rpl20

LSC · 72294-72644 · 351 bp

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Pi 0.0171

MarkerSeek score 49.90

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 351
variable_sites: 9
pi: 0.0171
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	AAGAGGGGAATCGATCGCTA	AGTAGCAAAAGCCGTTGGAA	50.0	45.0	58.0	58.0	834-886 bp	1.0	85.8
rpl20_p2	AGGGGAATCGATCGCTAGAA	AGTAGCAAAAGCCGTTGGAA	50.0	45.0	58.0	58.0	831-883 bp	1.0	85.8
rpl20_p3	AAGAGGGGAATCGATCGCTA	TGGAACCCACTAGTTAGCCA	50.0	50.0	58.0	58.0	974-1023 bp	1.0	85.0
rpl20_p4	AGGGGAATCGATCGCTAGAA	TGGAACCCACTAGTTAGCCA	50.0	50.0	58.0	58.0	971-1020 bp	1.0	85.0
rpl20_p5	TCCAATCGGGGGATCGAATA	AGTAGCAAAAGCCGTTGGAA	50.0	45.0	58.0	58.0	1024-1076 bp	1.0	84.6