Hotspot

ndhF

SSC · 130396-131001 · 606 bp

Back to Edgeworthia

Pi 0.0446

MarkerSeek score 72.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 606
variable_sites: 40
pi: 0.0446
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAAAGCGGGCTACAAGAAA	AAAAGCTTTCAACGCTGCTC	45.0	45.0	58.0	57.9	1282 bp	0.333333	52.1
ndhF_p2	AAGGGGAAATTGTGCGGATT	AAAAGCTTTCAACGCTGCTC	45.0	45.0	58.1	57.9	1178 bp	0.333333	51.8
ndhF_p3	AGGGGAAATTGTGCGGATTT	AAAAGCTTTCAACGCTGCTC	45.0	45.0	58.1	57.9	1177 bp	0.333333	51.8
ndhF_p4	GATCCCATGCCCAAAGCTAA	AAAAGCTTTCAACGCTGCTC	50.0	45.0	57.9	57.9	1453 bp	0.333333	51.8
ndhF_p5	TAAGATCCCATGCCCAAAGC	AAAAGCTTTCAACGCTGCTC	50.0	45.0	57.9	57.9	1456 bp	0.333333	51.8