Hotspot

accD-psaI

LSC · 62262-63031 · 770 bp

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Pi 0.0254

MarkerSeek score 62.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 29
pi: 0.0254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGAGGTAGTATGGGATCCGT	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.7	58.0	1323-1327 bp	1.0	80.0
accD-psaI_p2	GCCTATATTGCATTTGCGGG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	1044-1048 bp	1.0	80.0
accD-psaI_p3	TGGGATCCGTAGTAGGTGAG	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.6	58.0	1313-1317 bp	1.0	79.6
accD-psaI_p4	GGAGGTAGTATGGGATCCGT	TCAGATTTTGTCTTGGCCCC	55.0	50.0	57.7	58.1	1391-1395 bp	1.0	79.5
accD-psaI_p5	GGAGGTAGTATGGGATCCGT	AAGAAGCCATTGCAATTGCC	55.0	45.0	57.7	57.9	1329-1333 bp	1.0	79.4