Hotspot

ndhK-ndhC

LSC · 53404-53474 · 71 bp

Back to Edgeworthia

Pi 0.0798

MarkerSeek score 73.40

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 8
pi: 0.0798
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	TCAATGAAGCAACAGCTGGT	CATGGCGAAAAGGAGCATTG	45.0	50.0	58.0	58.1	243-245 bp	1.0	82.2
ndhK-ndhC_p2	TCAACTCCCCGAACAGTACT	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	483-485 bp	1.0	80.8
ndhK-ndhC_p3	TCAATGAAGCAACAGCTGGT	TACGAAGTGAGGTGAAAGCG	45.0	50.0	58.0	57.9	904-906 bp	0.666667	67.0
ndhK-ndhC_p4	TCAACTCCCCGAACAGTACT	TACGAAGTGAGGTGAAAGCG	50.0	50.0	58.0	57.9	1144-1146 bp	0.666667	66.9
ndhK-ndhC_p5	TCTCACTAAAGAAGGGGCCA	TACGAAGTGAGGTGAAAGCG	50.0	50.0	58.0	57.9	1037-1039 bp	0.666667	66.7