Hotspot

trnL-UAA-trnF-GAA

LSC · 51191-51570 · 380 bp

Back to Edgeworthia

Pi 0.0161

MarkerSeek score 68.50

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 9
pi: 0.0161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	470-497 bp	1.0	79.8
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	432-459 bp	1.0	76.9
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	467-494 bp	1.0	76.5
trnL-UAA-trnF-GAA_p4	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	459-486 bp	1.0	75.9
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TGCCGAGAACCAGATTTGAA	50.0	45.0	57.5	57.4	484-511 bp	1.0	75.6