Hotspot

rps4-trnT-UGU

LSC · 49073-49558 · 486 bp

Back to Edgeworthia

Pi 0.0279

MarkerSeek score 66.80

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 486
variable_sites: 20
pi: 0.0279
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	TTTAAGCCCGCTTAGCTCAG	50.0	50.0	58.0	58.0	653-661 bp	1.0	83.0
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	TTTAAGCCCGCTTAGCTCAG	50.0	50.0	58.1	58.0	657-665 bp	1.0	82.7
rps4-trnT-UGU_p3	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	638-646 bp	1.0	82.2
rps4-trnT-UGU_p4	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	642-650 bp	1.0	81.9
rps4-trnT-UGU_p5	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	606-614 bp	1.0	81.6