Hotspot

ycf3-trnS-GGA

LSC · 47114-48039 · 926 bp

Back to Edgeworthia

Pi 0.0137

MarkerSeek score 70.90

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 926
variable_sites: 18
pi: 0.0137
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	TGAATTAGGTGAAGGTGCGG	47.6	50.0	58.0	57.9	1049-1052 bp	1.0	74.4
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	983-986 bp	1.0	74.0
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TTAGGTGAAGGTGCGGAAAG	47.6	50.0	58.0	57.8	1045-1048 bp	1.0	73.8
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	GTGCGGAAAGAGAGGGATTC	47.6	55.0	58.0	58.3	1035-1038 bp	1.0	72.6
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	GAATTAGGTGAAGGTGCGGA	47.6	50.0	58.0	57.6	1048-1051 bp	1.0	72.1