Hotspot

rps16-trnQ-UUG

LSC · 6303-7562 · 1260 bp

Back to Edgeworthia

Pi 0.0152
MarkerSeek score 53.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1260
variable_sites
20
pi
0.0152
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.87
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA AAGGTTCGAATCCTTCCGTC 45.0 50.0 58.1 57.6 668-1333 bp 1.0 81.3
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC AAGGTTCGAATCCTTCCGTC 50.0 50.0 58.2 57.6 679-1344 bp 1.0 80.8
rps16-trnQ-UUG_p3 ATCATGTCCTTCAAGTCGCA AAGGTTCGAATCCTTCCGTC 45.0 50.0 57.2 57.6 680-1345 bp 1.0 76.3
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG AAGGTTCGAATCCTTCCGTC 50.0 50.0 57.2 57.6 660-1325 bp 1.0 76.2
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GTTCGAATCCTTCCGTCCC 45.0 57.9 58.1 57.9 665-1330 bp 1.0 75.9