Hotspot

psbA-trnK-UUU

LSC · 1529-1812 · 284 bp

Back to Edgeworthia

Pi 0.0122

MarkerSeek score 64.90

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 284
variable_sites: 5
pi: 0.0122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AACACACCAAACCATCCGAT	CGGTTTGGGGAGGGATTTTT	45.0	50.0	57.7	58.4	475-485 bp	1.0	78.8
psbA-trnK-UUU_p2	AACACACCAAACCATCCGAT	AAAATGCAAGCACGGTTTGG	45.0	45.0	57.7	58.4	487-497 bp	1.0	78.3
psbA-trnK-UUU_p3	ACACACCAAACCATCCGATG	CGGTTTGGGGAGGGATTTTT	50.0	50.0	58.5	58.4	474-484 bp	1.0	77.4
psbA-trnK-UUU_p4	CACACCAAACCATCCGATGT	CGGTTTGGGGAGGGATTTTT	50.0	50.0	58.5	58.4	473-483 bp	1.0	77.4
psbA-trnK-UUU_p5	AACACACCAAACCATCCGAT	TGAAAAATGCAAGCACGGTT	45.0	40.0	57.7	57.4	490-500 bp	1.0	76.9