Hotspot

rps2

LSC · 23443-24153 · 711 bp

Back to Eclipta

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 1
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	GGCTGTGTATCTACGCCAAT	AGTCAGAAATAGTAACCGATTCGA	50.0	37.5	57.8	57.6	896 bp	1.0	42.6
rps2_p2	TTCAAAAGGGAGAGGCAGTG	AGTCAGAAATAGTAACCGATTCGA	50.0	37.5	57.7	57.6	814 bp	1.0	42.3
rps2_p3	GGCTGTGTATCTACGCCAAT	TGAGAGTCAGAAATAGTAACCGA	50.0	39.1	57.8	56.5	900 bp	1.0	42.3
rps2_p4	TTCAAAAGGGAGAGGCAGTG	TGAGAGTCAGAAATAGTAACCGA	50.0	39.1	57.7	56.5	818 bp	1.0	42.0
rps2_p5	GGCTGTGTATCTACGCCAAT	TCTATGAAGGTTCCGAAATGA	50.0	38.1	57.8	54.4	868 bp	1.0	41.1