Hotspot

rpl16

LSC · 80361-81790 · 1430 bp

Back to Eclipta

Pi 0.000702

MarkerSeek score 59.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1430
variable_sites: 1
pi: 0.000702
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AAAAGAAACCTGCACCGCTA	ATGAATATTTTGATCAAATTGCC	45.0	26.1	58.0	52.4	1488-1494 bp	1.0	40.4
rpl16_p2	AAAAGAAACCTGCACCGCTA	AATGAATATTTTGATCAAATTGCC	45.0	25.0	58.0	53.2	1489-1495 bp	1.0	40.4
rpl16_p3	AAAGAAACCTGCACCGCTAT	ATGAATATTTTGATCAAATTGCC	45.0	26.1	57.8	52.4	1487-1493 bp	1.0	40.4
rpl16_p4	AAGAAACCTGCACCGCTATT	ATGAATATTTTGATCAAATTGCC	45.0	26.1	57.8	52.4	1486-1492 bp	1.0	40.4
rpl16_p5	AGAAACCTGCACCGCTATTT	ATGAATATTTTGATCAAATTGCC	45.0	26.1	57.8	52.4	1485-1491 bp	1.0	40.4