Hotspot

clpP

LSC · 69090-71124 · 2035 bp

Back to Eclipta

Pi 0.000991

MarkerSeek score 59.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2035
variable_sites: 2
pi: 0.000991
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AAACTCGGCAGGGTGAATTT	AGCTTTGTTACGTTTCCACA	45.0	40.0	58.0	55.8	2128-2144 bp	1.0	63.2
clpP_p2	AAACTCGGCAGGGTGAATTT	GCTTTGTTACGTTTCCACATCA	45.0	40.9	58.0	57.8	2127-2143 bp	1.0	62.9
clpP_p3	AAACTCGGCAGGGTGAATTT	AAGCTTTGTTACGTTTCCACA	45.0	38.1	58.0	56.6	2129-2145 bp	1.0	61.4
clpP_p4	AAACTCGGCAGGGTGAATTT	AGCTTTGTTACGTTTCCACATC	45.0	40.9	58.0	57.5	2128-2144 bp	1.0	60.8
clpP_p5	AAACTCGGCAGGGTGAATTT	AGCTTTGTTACGTTTCCACAT	45.0	38.1	58.0	56.4	2128-2144 bp	1.0	59.7