Hotspot

atpF

LSC · 26900-28155 · 1256 bp

Back to Eclipta

Pi 0.00159
MarkerSeek score 59.70
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1256
variable_sites
2
pi
0.00159
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 ACAAAAAGAACTCTGTTCGA ATTTCGTCGGCTTGAATGGT 35.0 45.0 53.0 58.2 1391 bp 1.0 40.1
atpF_p2 ACAAAAAGAACTCTGTTCGA GTCGGCTTGAATGGTTACCA 35.0 50.0 53.0 58.2 1386 bp 1.0 40.1
atpF_p3 ACAAAAAGAACTCTGTTCGA TCGTCGGCTTGAATGGTTAC 35.0 50.0 53.0 58.3 1388 bp 1.0 40.1
atpF_p4 ACAAAAAGAACTCTGTTCGA TTTCGTCGGCTTGAATGGTT 35.0 45.0 53.0 58.4 1390 bp 1.0 40.1
atpF_p5 ACAAAAAGAACTCTGTTCGA TTGCTCAATACGTTCACGGA 35.0 45.0 53.0 57.6 1422 bp 1.0 40.1