Hotspot

ndhD-psaC

SSC · 115521-115637 · 117 bp

Back to Eclipta

Pi 0.0256

MarkerSeek score 67.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 117
variable_sites: 3
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGGAACCTGCAAATATTGGT	ACAGAGGATTGTGTCGGTTG	40.0	50.0	54.9	57.8	289 bp	1.0	54.5
ndhD-psaC_p2	AGGAACCTGCAAATATTGGT	CAGAGGATTGTGTCGGTTGT	40.0	50.0	54.9	57.8	288 bp	1.0	54.5
ndhD-psaC_p3	AGGAACCTGCAAATATTGGT	TGCCCAACAGACTTCTTGAG	40.0	50.0	54.9	57.7	250 bp	1.0	53.9
ndhD-psaC_p4	AGGAACCTGCAAATATTGGT	GCCCAACAGACTTCTTGAGT	40.0	50.0	54.9	57.7	249 bp	1.0	53.9
ndhD-psaC_p5	AGGAACCTGCAAATATTGGT	AAGAGATGTGAATCCGCCTG	40.0	50.0	54.9	57.7	268 bp	1.0	53.3