Hotspot

rpl32-trnL-UAG

SSC · 111796-112570 · 775 bp

Back to Eclipta

Pi 0.00776
MarkerSeek score 63.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
775
variable_sites
6
pi
0.00776
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAGAAAGCGTATGGGGTGG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 907-908 bp 1.0 79.3
rpl32-trnL-UAG_p2 AAAGAAAGCGTATGGGGTGG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 908-909 bp 1.0 79.3
rpl32-trnL-UAG_p3 GGGTGGCGTTAAAAGCTTTG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 893-894 bp 1.0 78.9
rpl32-trnL-UAG_p4 GGGTGGCGTTAAAAGCTTTG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 894-895 bp 1.0 78.9
rpl32-trnL-UAG_p5 AAAGAAAGCGTATGGGGTGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 917-918 bp 1.0 78.4