Hotspot

ndhF-rpl32

SSC · 110618-111630 · 1013 bp

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Pi 0.00494

MarkerSeek score 61.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1013
variable_sites: 5
pi: 0.00494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CCACCCCATACGCTTTCTTT	50.0	50.0	58.0	58.2	1193 bp	1.0	78.7
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CCACCCCATACGCTTTCTTT	45.0	50.0	58.0	58.2	1191 bp	1.0	78.7
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CCACCCCATACGCTTTCTTT	45.0	50.0	58.0	58.2	1192 bp	1.0	78.7
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	CAAAGCTTTTAACGCCACCC	50.0	50.0	58.0	58.2	1207 bp	1.0	78.3
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	CAAAGCTTTTAACGCCACCC	45.0	50.0	58.0	58.2	1205 bp	1.0	78.2