primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	trnS-GCU-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	8637	9386	900-901	1.000000	78.5
trnS-GCU-trnC-GCA_p2	trnS-GCU-trnC-GCA	2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	8637	9386	882-883	1.000000	76.3
trnS-GCU-trnC-GCA_p3	trnS-GCU-trnC-GCA	3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.718384	8637	9386	836-837	1.000000	74.6
trnS-GCU-trnC-GCA_p4	trnS-GCU-trnC-GCA	4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.756923	8637	9386	901-902	1.000000	74.3
trnS-GCU-trnC-GCA_p5	trnS-GCU-trnC-GCA	5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.6	58.4	0.0	14.6	0.991747	8637	9386	818-819	1.000000	72.5
rps2_p1	rps2	1	GGCTGTGTATCTACGCCAAT	AGTCAGAAATAGTAACCGATTCGA	20	24	50.0	37.5	57.8	57.6	0.0	7.8	4.687275	23443	24153	896-896	1.000000	42.6
rps2_p2	rps2	2	TTCAAAAGGGAGAGGCAGTG	AGTCAGAAATAGTAACCGATTCGA	20	24	50.0	37.5	57.7	57.6	0.0	7.8	4.723513	23443	24153	814-814	1.000000	42.3
rps2_p3	rps2	3	GGCTGTGTATCTACGCCAAT	TGAGAGTCAGAAATAGTAACCGA	20	23	50.0	39.1	57.8	56.5	0.0	0.0	4.728284	23443	24153	900-900	1.000000	42.3
rps2_p4	rps2	4	TTCAAAAGGGAGAGGCAGTG	TGAGAGTCAGAAATAGTAACCGA	20	23	50.0	39.1	57.7	56.5	0.0	0.0	4.764523	23443	24153	818-818	1.000000	42.0
rps2_p5	rps2	5	GGCTGTGTATCTACGCCAAT	TCTATGAAGGTTCCGAAATGA	20	21	50.0	38.1	57.8	54.4	0.0	0.0	4.871158	23443	24153	868-868	1.000000	41.1
atpF_p1	atpF	1	ACAAAAAGAACTCTGTTCGA	ATTTCGTCGGCTTGAATGGT	20	20	35.0	45.0	53.0	58.2	5.0	0.0	5.161956	26900	28155	1391-1391	1.000000	40.1
atpF_p2	atpF	2	ACAAAAAGAACTCTGTTCGA	GTCGGCTTGAATGGTTACCA	20	20	35.0	50.0	53.0	58.2	5.0	6.5	5.167098	26900	28155	1386-1386	1.000000	40.1
atpF_p3	atpF	3	ACAAAAAGAACTCTGTTCGA	TCGTCGGCTTGAATGGTTAC	20	20	35.0	50.0	53.0	58.3	5.0	0.0	5.265979	26900	28155	1388-1388	1.000000	40.1
atpF_p4	atpF	4	ACAAAAAGAACTCTGTTCGA	TTTCGTCGGCTTGAATGGTT	20	20	35.0	45.0	53.0	58.4	5.0	0.0	5.383149	26900	28155	1390-1390	1.000000	40.1
atpF_p5	atpF	5	ACAAAAAGAACTCTGTTCGA	TTGCTCAATACGTTCACGGA	20	20	35.0	45.0	53.0	57.6	5.0	4.1	5.425385	26900	28155	1422-1422	1.000000	40.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	46273	46847	618-625	1.000000	80.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	46273	46847	626-633	1.000000	79.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	46273	46847	650-657	1.000000	79.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	46273	46847	658-665	1.000000	78.7
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	46273	46847	632-639	1.000000	78.3
atpB-rbcL_p1	atpB-rbcL	1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.047103	53431	54198	923-929	1.000000	72.2
atpB-rbcL_p2	atpB-rbcL	2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	58.0	58.1	0.0	0.0	1.080391	53431	54198	922-928	1.000000	72.0
atpB-rbcL_p3	atpB-rbcL	3	GGCAACCCCAGAACTAGAAG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	57.9	58.0	0.0	0.0	1.132608	53431	54198	850-856	1.000000	71.6
atpB-rbcL_p4	atpB-rbcL	4	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.141542	53431	54198	880-886	1.000000	71.5
atpB-rbcL_p5	atpB-rbcL	5	GGCAACCCCAGAACTAGAAG	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	57.9	58.1	0.0	0.0	1.165895	53431	54198	849-855	1.000000	71.3
ycf4-cemA_p1	ycf4-cemA	1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.106483	59485	60309	1005-1005	1.000000	79.3
ycf4-cemA_p2	ycf4-cemA	2	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.172357	59485	60309	969-969	1.000000	78.8
ycf4-cemA_p3	ycf4-cemA	3	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	59485	60309	937-937	1.000000	78.8
ycf4-cemA_p4	ycf4-cemA	4	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.251008	59485	60309	951-951	1.000000	78.2
ycf4-cemA_p5	ycf4-cemA	5	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.297297	59485	60309	949-949	1.000000	77.8
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	63756	65049	1486-1486	1.000000	72.2
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	24.9	0.0	1.097796	63756	65049	1409-1409	1.000000	71.3
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.2	57.1	0.0	0.0	1.112474	63756	65049	1495-1495	1.000000	71.2
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	58.2	57.1	0.0	0.0	1.125378	63756	65049	1500-1500	1.000000	71.1
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.8	57.1	0.0	0.0	1.128804	63756	65049	1494-1494	1.000000	71.1
clpP_p1	clpP	1	AAACTCGGCAGGGTGAATTT	AGCTTTGTTACGTTTCCACA	20	20	45.0	40.0	58.0	55.8	0.0	0.0	2.186096	69090	71124	2128-2144	1.000000	63.2
clpP_p2	clpP	2	AAACTCGGCAGGGTGAATTT	GCTTTGTTACGTTTCCACATCA	20	22	45.0	40.9	58.0	57.8	0.0	0.0	2.222377	69090	71124	2127-2143	1.000000	62.9
clpP_p3	clpP	3	AAACTCGGCAGGGTGAATTT	AAGCTTTGTTACGTTTCCACA	20	21	45.0	38.1	58.0	56.6	0.0	0.0	2.413528	69090	71124	2129-2145	1.000000	61.4
clpP_p4	clpP	4	AAACTCGGCAGGGTGAATTT	AGCTTTGTTACGTTTCCACATC	20	22	45.0	40.9	58.0	57.5	0.0	0.0	2.488100	69090	71124	2128-2144	1.000000	60.8
clpP_p5	clpP	5	AAACTCGGCAGGGTGAATTT	AGCTTTGTTACGTTTCCACAT	20	21	45.0	38.1	58.0	56.4	0.0	0.0	2.631977	69090	71124	2128-2144	1.000000	59.7
petD_p1	petD	1	ATTTCCCTTGAACTCCGCAA	TGAAGCGACTGTTCCCTAGA	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.367124	75695	76907	1347-1354	1.000000	77.2
petD_p2	petD	2	TTTCCCTTGAACTCCGCAAT	TGAAGCGACTGTTCCCTAGA	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.367124	75695	76907	1346-1353	1.000000	77.2
petD_p3	petD	3	ATTTCCCTTGAACTCCGCAA	CTTTGAAGCGACTGTTCCCT	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.402129	75695	76907	1350-1357	1.000000	76.9
petD_p4	petD	4	TTTCCCTTGAACTCCGCAAT	CTTTGAAGCGACTGTTCCCT	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.402129	75695	76907	1349-1356	1.000000	76.9
petD_p5	petD	5	TTTCCCTTGAACTCCGCAAT	TCGGAATCAGCATAGGTCCA	20	20	45.0	50.0	57.7	58.2	0.0	0.0	0.498136	75695	76907	1405-1412	1.000000	76.1
rpoA-rps11_p1	rpoA-rps11	1	ACTTCCACTGTAGTGTCCGA	AAGAGACGCAGCATTACGAG	20	20	50.0	50.0	58.0	58.1	10.8	0.0	0.094245	78113	78192	244-244	1.000000	79.6
rpoA-rps11_p2	rpoA-rps11	2	TTCCACTGTAGTGTCCGAGT	AAGAGACGCAGCATTACGAG	20	20	50.0	50.0	58.0	58.1	10.8	0.0	0.094245	78113	78192	242-242	1.000000	79.6
rpoA-rps11_p3	rpoA-rps11	3	ACTTCCACTGTAGTGTCCGA	GAAGAGACGCAGCATTACGA	20	20	50.0	50.0	58.0	58.1	10.8	0.0	0.099503	78113	78192	245-245	1.000000	79.5
rpoA-rps11_p4	rpoA-rps11	4	TTCCACTGTAGTGTCCGAGT	GAAGAGACGCAGCATTACGA	20	20	50.0	50.0	58.0	58.1	10.8	0.0	0.099503	78113	78192	243-243	1.000000	79.5
rpoA-rps11_p5	rpoA-rps11	5	GCTTACTGTCTGCTGCTGAT	AAGAGACGCAGCATTACGAG	20	20	50.0	50.0	58.0	58.1	12.4	0.0	0.106722	78113	78192	271-271	1.000000	79.4
rpl16_p1	rpl16	1	AAAAGAAACCTGCACCGCTA	ATGAATATTTTGATCAAATTGCC	20	23	45.0	26.1	58.0	52.4	0.0	21.1	8.643051	80361	81790	1488-1494	1.000000	40.4
rpl16_p2	rpl16	2	AAAAGAAACCTGCACCGCTA	AATGAATATTTTGATCAAATTGCC	20	24	45.0	25.0	58.0	53.2	0.0	21.1	8.817184	80361	81790	1489-1495	1.000000	40.4
rpl16_p3	rpl16	3	AAAGAAACCTGCACCGCTAT	ATGAATATTTTGATCAAATTGCC	20	23	45.0	26.1	57.8	52.4	0.0	21.1	8.819083	80361	81790	1487-1493	1.000000	40.4
rpl16_p4	rpl16	4	AAGAAACCTGCACCGCTATT	ATGAATATTTTGATCAAATTGCC	20	23	45.0	26.1	57.8	52.4	0.0	21.1	8.819083	80361	81790	1486-1492	1.000000	40.4
rpl16_p5	rpl16	5	AGAAACCTGCACCGCTATTT	ATGAATATTTTGATCAAATTGCC	20	23	45.0	26.1	57.8	52.4	0.0	21.1	8.819083	80361	81790	1485-1491	1.000000	40.4
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	CCACCCCATACGCTTTCTTT	20	20	50.0	50.0	58.0	58.2	17.5	0.0	0.198226	110618	111630	1193-1193	1.000000	78.7
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	CCACCCCATACGCTTTCTTT	20	20	45.0	50.0	58.0	58.2	17.5	0.0	0.209515	110618	111630	1191-1191	1.000000	78.7
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	CCACCCCATACGCTTTCTTT	20	20	45.0	50.0	58.0	58.2	17.5	0.0	0.209515	110618	111630	1192-1192	1.000000	78.7
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	CAAAGCTTTTAACGCCACCC	20	20	50.0	50.0	58.0	58.2	17.5	9.5	0.254080	110618	111630	1207-1207	1.000000	78.3
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	CAAAGCTTTTAACGCCACCC	20	20	45.0	50.0	58.0	58.2	17.5	9.5	0.265369	110618	111630	1205-1205	1.000000	78.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAAGAAAGCGTATGGGGTGG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.173642	111796	112570	907-908	1.000000	79.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAAGAAAGCGTATGGGGTGG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.175013	111796	112570	908-909	1.000000	79.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGTGGCGTTAAAAGCTTTG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	13.5	0.0	0.229496	111796	112570	893-894	1.000000	78.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGTGGCGTTAAAAGCTTTG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	13.5	0.0	0.230866	111796	112570	894-895	1.000000	78.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAAGAAAGCGTATGGGGTGG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.290089	111796	112570	917-918	1.000000	78.4
ndhD_p1	ndhD	1	TGCCAACCAACTCTTAAAAA	GGCACAGGTTTTTCTGGTCT	20	20	35.0	50.0	53.5	58.3	0.0	0.0	4.815552	114018	115520	1583-1583	1.000000	41.5
ndhD_p2	ndhD	2	TTGCCAACCAACTCTTAAAA	GGCACAGGTTTTTCTGGTCT	20	20	35.0	50.0	53.5	58.3	0.0	0.0	4.815552	114018	115520	1584-1584	1.000000	41.5
ndhD_p3	ndhD	3	TTTGCCAACCAACTCTTAAA	GGCACAGGTTTTTCTGGTCT	20	20	35.0	50.0	53.5	58.3	0.0	0.0	4.815552	114018	115520	1585-1585	1.000000	41.5
ndhD_p4	ndhD	4	TTTTGCCAACCAACTCTTAA	GGCACAGGTTTTTCTGGTCT	20	20	35.0	50.0	53.5	58.3	0.0	0.0	4.815552	114018	115520	1586-1586	1.000000	41.5
ndhD_p5	ndhD	5	TTTTTGCCAACCAACTCTTA	GGCACAGGTTTTTCTGGTCT	20	20	35.0	50.0	53.5	58.3	0.0	0.0	4.815552	114018	115520	1587-1587	1.000000	41.5
ndhD-psaC_p1	ndhD-psaC	1	AGGAACCTGCAAATATTGGT	ACAGAGGATTGTGTCGGTTG	20	20	40.0	50.0	54.9	57.8	0.0	0.0	3.292374	115521	115637	289-289	1.000000	54.5
ndhD-psaC_p2	ndhD-psaC	2	AGGAACCTGCAAATATTGGT	CAGAGGATTGTGTCGGTTGT	20	20	40.0	50.0	54.9	57.8	0.0	0.0	3.292374	115521	115637	288-288	1.000000	54.5
ndhD-psaC_p3	ndhD-psaC	3	AGGAACCTGCAAATATTGGT	TGCCCAACAGACTTCTTGAG	20	20	40.0	50.0	54.9	57.7	0.0	0.0	3.383386	115521	115637	250-250	1.000000	53.9
ndhD-psaC_p4	ndhD-psaC	4	AGGAACCTGCAAATATTGGT	GCCCAACAGACTTCTTGAGT	20	20	40.0	50.0	54.9	57.7	0.0	0.0	3.384965	115521	115637	249-249	1.000000	53.9
ndhD-psaC_p5	ndhD-psaC	5	AGGAACCTGCAAATATTGGT	AAGAGATGTGAATCCGCCTG	20	20	40.0	50.0	54.9	57.7	0.0	0.0	3.444897	115521	115637	268-268	1.000000	53.3
ndhA_p1	ndhA	1	TGTTTTGGACAAGTCGTGAA	GGCCGCCGGGATTTATTAAT	20	20	40.0	50.0	55.8	58.5	0.0	4.8	2.707775	118124	120241	2273-2273	1.000000	58.4
ndhA_p2	ndhA	2	TTGTTTTGGACAAGTCGTGA	GGCCGCCGGGATTTATTAAT	20	20	40.0	50.0	55.8	58.5	0.0	4.8	2.707775	118124	120241	2274-2274	1.000000	58.4
ndhA_p3	ndhA	3	TTGTTTTGGACAAGTCGTGAA	GGCCGCCGGGATTTATTAAT	21	20	38.1	50.0	56.5	58.5	0.0	4.8	2.932677	118124	120241	2274-2274	1.000000	56.6
ndhA_p4	ndhA	4	TGTTTTGGACAAGTCGTGAAT	GGCCGCCGGGATTTATTAAT	21	20	38.1	50.0	56.3	58.5	0.0	4.8	3.151188	118124	120241	2273-2273	1.000000	54.9
ndhA_p5	ndhA	5	TGTTTTGGACAAGTCGTGAA	GGCCGCCGGGATTTATTAA	20	19	40.0	52.6	55.8	58.0	0.0	4.8	3.273585	118124	120241	2273-2273	1.000000	53.9
ycf1_p1	ycf1	1	TCATCCAAAAATTGACTCCAGT	AATTATGGAACCTGCCCGTC	22	20	36.4	50.0	55.9	57.9	0.0	0.0	4.161534	122451	122570	351-351	1.000000	47.2
ycf1_p2	ycf1	2	TCATCCAAAAATTGACTCCAGT	TATGGAACCTGCCCGTCTAT	22	20	36.4	50.0	55.9	57.9	0.0	0.0	4.205862	122451	122570	348-348	1.000000	46.8
ycf1_p3	ycf1	3	TCATCCAAAAATTGACTCCAGT	TTATGGAACCTGCCCGTCTA	22	20	36.4	50.0	55.9	58.1	0.0	0.0	4.240039	122451	122570	349-349	1.000000	46.5
ycf1_p4	ycf1	4	TCATCCAAAAATTGACTCCAGT	AACCTGCCCGTCTATCTGTA	22	20	36.4	50.0	55.9	57.8	0.0	0.0	4.263319	122451	122570	343-343	1.000000	46.4
ycf1_p5	ycf1	5	TCATCCAAAAATTGACTCCAGT	ACCTGCCCGTCTATCTGTAA	22	20	36.4	50.0	55.9	57.8	0.0	0.0	4.263319	122451	122570	342-342	1.000000	46.4