Hotspot

ycf34

LSC · 48750-49034 · 285 bp

Back to Ecklonia

Pi 0.014

MarkerSeek score 48.10

Primer available yes

Label ycf34

Feature Metadata

label_name: ycf34
feature_type: gene
region: LSC
length_bp: 285
variable_sites: 6
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf34_p1	ATGGTTCAGATGCTGCTAGC	TGCAAATCGTGAGGGTTCAA	50.0	45.0	58.0	58.0	1042-1054 bp	1.0	81.0
ycf34_p2	GAAGTAACAGCCGATCCTGG	TGCAAATCGTGAGGGTTCAA	55.0	45.0	58.1	58.0	830-842 bp	1.0	81.0
ycf34_p3	GATCCTGGAAAAATTGCGGC	TGCAAATCGTGAGGGTTCAA	50.0	45.0	58.1	58.0	818-830 bp	1.0	80.9
ycf34_p4	GCCGATCCTGGAAAAATTGC	TGCAAATCGTGAGGGTTCAA	50.0	45.0	58.1	58.0	821-833 bp	1.0	80.9
ycf34_p5	CTGGAAAAATTGCGGCTCTG	TGCAAATCGTGAGGGTTCAA	50.0	45.0	58.0	58.0	814-826 bp	0.666667	67.5