Hotspot

rps20

LSC · 24233-24538 · 306 bp

Back to Ecklonia

Pi 0.0196

MarkerSeek score 52.40

Primer available yes

Label rps20

Feature Metadata

label_name: rps20
feature_type: gene
region: LSC
length_bp: 306
variable_sites: 9
pi: 0.0196
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps20_p1	TATCGAACCGTTCTTCACCG	TGGTCGATTGCGTCTGATTT	50.0	45.0	57.7	57.9	901-908 bp	1.0	79.8
rps20_p2	TATCGAACCGTTCTTCACCG	GATGGTCGATTGCGTCTGAT	50.0	50.0	57.7	58.1	903-910 bp	1.0	79.4
rps20_p3	AACCGTTCTTCACCGTTTCT	TGGTCGATTGCGTCTGATTT	45.0	45.0	57.7	57.9	896-903 bp	1.0	79.3
rps20_p4	ACCGTTCTTCACCGTTTCTT	TGGTCGATTGCGTCTGATTT	45.0	45.0	57.7	57.9	895-902 bp	1.0	79.3
rps20_p5	TATCGAACCGTTCTTCACCG	AGAACAATGCCTTGCCCTAG	50.0	50.0	57.7	57.9	1327-1334 bp	1.0	79.1