Hotspot

petF

SSC · 108341-108634 · 294 bp

Back to Ecklonia

Pi 0.051

MarkerSeek score 75.70

Primer available yes

Label petF

Feature Metadata

label_name: petF
feature_type: gene
region: SSC
length_bp: 294
variable_sites: 22
pi: 0.051
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petF_p1	ACACGGTACCATGGTAATGC	GATGAACCAAGGTGGCTCAA	50.0	50.0	58.0	58.1	901-919 bp	0.666667	67.7
petF_p2	TGACGCCACATTGGGTTTAA	GATGAACCAAGGTGGCTCAA	45.0	50.0	58.0	58.1	1051-1069 bp	0.666667	67.5
petF_p3	TTGACGCCACATTGGGTTTA	GATGAACCAAGGTGGCTCAA	45.0	50.0	58.0	58.1	1052-1070 bp	0.666667	67.5
petF_p4	TGCCAAACAGCAGCTAAGAA	GATGAACCAAGGTGGCTCAA	45.0	50.0	58.0	58.1	1222-1240 bp	0.666667	67.3
petF_p5	ACCCTGTCCATGAGTCAGAA	GATGAACCAAGGTGGCTCAA	50.0	50.0	58.0	58.1	1110-1128 bp	0.666667	67.3