Hotspot

ycf16-trnN

SSC · 120832-121102 · 271 bp

Back to Ecklonia

Pi 0.0517

MarkerSeek score 75.80

Primer available yes

Label ycf16-trnN

Feature Metadata

label_name: ycf16-trnN
feature_type: igs
region: SSC
length_bp: 271
variable_sites: 21
pi: 0.0517
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf16-trnN_p1	TCATGTTATGCAAGATGGCCA	AAGCTCCCCAGGTAGGATTT	42.9	50.0	58.0	58.0	449 bp	1.0	75.5
ycf16-trnN_p2	ACAGGTGATGCCAATTTAGCA	AAGCTCCCCAGGTAGGATTT	42.9	50.0	58.2	58.0	418 bp	1.0	74.4
ycf16-trnN_p3	TCATGTTATGCAAGATGGCCA	CCGATTGCTCTACCACTGAG	42.9	55.0	58.0	57.8	404 bp	1.0	74.3
ycf16-trnN_p4	ACAGGTGATGCCAATTTAGCA	CCGATTGCTCTACCACTGAG	42.9	55.0	58.2	57.8	373 bp	1.0	73.2
ycf16-trnN_p5	TCATGTTATGCAAGATGGCCA	CCCCAGGTAGGATTTGAACC	42.9	55.0	58.0	57.6	444 bp	1.0	72.2