Hotspot

ftsH-psbH

SSC · 105847-105958 · 112 bp

Back to Ecklonia

Pi 0.0833

MarkerSeek score 75.60

Primer available yes

Label ftsH-psbH

Feature Metadata

label_name: ftsH-psbH
feature_type: igs
region: SSC
length_bp: 112
variable_sites: 14
pi: 0.0833
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsH-psbH_p1	TGTTTCCTTGATGTTGCGGA	CCGGTCCAAATGCCGTATAA	45.0	50.0	58.0	58.0	705-706 bp	1.0	81.4
ftsH-psbH_p2	GGGCGACGTTTACAAACAAG	CCGGTCCAAATGCCGTATAA	50.0	50.0	58.0	58.0	732-733 bp	1.0	81.3
ftsH-psbH_p3	CCAGTTTAACCCCAATGGCT	CCGGTCCAAATGCCGTATAA	50.0	50.0	58.1	58.0	782-783 bp	1.0	81.0
ftsH-psbH_p4	TGTTTCCTTGATGTTGCGGA	AATTTTGACCCCGGTCCAAA	45.0	45.0	58.0	57.9	715-716 bp	1.0	81.0
ftsH-psbH_p5	TCCAGTTTAACCCCAATGGC	CCGGTCCAAATGCCGTATAA	50.0	50.0	58.1	58.0	783-784 bp	1.0	80.9