Hotspot

psbY-rpl32

LSC · 81837-82175 · 339 bp

Back to Ecklonia

Pi 0.0308

MarkerSeek score 66.50

Primer available yes

Label psbY-rpl32

Feature Metadata

label_name: psbY-rpl32
feature_type: igs
region: LSC
length_bp: 339
variable_sites: 15
pi: 0.0308
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbY-rpl32_p1	TGATAGCAGCAGCATCTTGG	CTTCCCTTTGCTTTTGAGCG	50.0	50.0	58.0	57.9	444-460 bp	1.0	85.1
psbY-rpl32_p2	TGATAGCAGCAGCATCTTGG	CTTTGCTTTTGAGCGCCTTT	50.0	45.0	58.0	58.1	439-455 bp	1.0	84.5
psbY-rpl32_p3	TCAACAAATTAGCTTCGCGC	CTTCCCTTTGCTTTTGAGCG	45.0	50.0	57.8	57.9	387-403 bp	1.0	84.3
psbY-rpl32_p4	CTGTGATAGCAGCAGCATCT	CTTCCCTTTGCTTTTGAGCG	50.0	50.0	57.8	57.9	447-463 bp	1.0	83.4
psbY-rpl32_p5	TGATAGCAGCAGCATCTTGG	TTGCTTTTGAGCGCCTTTTT	50.0	40.0	58.0	57.7	437-453 bp	1.0	83.2