Hotspot

psbA-cbbx

LSC · 75146-75856 · 711 bp

Back to Ecklonia

Pi 0.0206

MarkerSeek score 61.20

Primer available yes

Label psbA-cbbx

Feature Metadata

label_name: psbA-cbbx
feature_type: igs
region: LSC
length_bp: 711
variable_sites: 22
pi: 0.0206
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-cbbx_p1	TGTTGCGATTTCTGCTCCTT	ACAGATGCGGATATTACAGCT	45.0	42.9	58.1	57.0	787 bp	1.0	65.1
psbA-cbbx_p2	TTCCTGTTGCGATTTCTGCT	ACAGATGCGGATATTACAGCT	45.0	42.9	58.1	57.0	791 bp	1.0	65.1
psbA-cbbx_p3	CTTCCTGTTGCGATTTCTGC	ACAGATGCGGATATTACAGCT	50.0	42.9	57.7	57.0	792 bp	1.0	63.9
psbA-cbbx_p4	GCGATTTCTGCTCCTTCTGT	ACAGATGCGGATATTACAGCT	50.0	42.9	58.3	57.0	783 bp	1.0	63.8
psbA-cbbx_p5	TGCGATTTCTGCTCCTTCTG	ACAGATGCGGATATTACAGCT	50.0	42.9	58.3	57.0	784 bp	1.0	63.8