Hotspot

psaJ-cp74

LSC · 63120-63310 · 191 bp

Back to Ecklonia

Pi 0.0392

MarkerSeek score 65.70

Primer available yes

Label psaJ-cp74

Feature Metadata

label_name: psaJ-cp74
feature_type: igs
region: LSC
length_bp: 191
variable_sites: 10
pi: 0.0392
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-cp74_p1	ATATGAGCCTGGGTCTCCTC	AAAAGTACCTGCTCTCGCTG	55.0	50.0	58.0	57.9	1160-1183 bp	1.0	82.1
psaJ-cp74_p2	GCCGATGTTGCAGGTTTAAC	AAAAGTACCTGCTCTCGCTG	50.0	50.0	58.0	57.9	1255-1278 bp	1.0	82.0
psaJ-cp74_p3	ATATGAGCCTGGGTCTCCTC	CCTCAACCTCAAGTGCATGT	55.0	50.0	58.0	58.1	1257-1280 bp	1.0	81.9
psaJ-cp74_p4	ATATGAGCCTGGGTCTCCTC	AAGGGTCTATTGCCTGGTCT	55.0	50.0	58.0	58.0	1230-1251 bp	0.666667	68.5
psaJ-cp74_p5	GCCGATGTTGCAGGTTTAAC	AAGGGTCTATTGCCTGGTCT	50.0	50.0	58.0	58.0	1325-1346 bp	0.666667	68.3