Hotspot

chlN-psaF

LSC · 62200-62323 · 124 bp

Back to Ecklonia

Pi 0.0437

MarkerSeek score 70.80

Primer available yes

Label chlN-psaF

Feature Metadata

label_name: chlN-psaF
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 8
pi: 0.0437
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN-psaF_p1	TGAAAAGATCGGCTCTCAGC	TCTGAATAGTAATGAAAAACTATGAGT	50.0	25.9	58.0	55.0	215-223 bp	1.0	43.7
chlN-psaF_p2	TGAAAAGATCGGCTCTCAGC	CTGAATAGTAATGAAAAACTATGAGT	50.0	26.9	58.0	53.7	214-222 bp	1.0	43.7
chlN-psaF_p3	GAAAAGATCGGCTCTCAGCT	TCTGAATAGTAATGAAAAACTATGAGT	50.0	25.9	57.7	55.0	214-222 bp	1.0	43.7
chlN-psaF_p4	TGAAAAGATCGGCTCTCAGC	TCTGAATAGTAATGAAAAACTATGAG	50.0	26.9	58.0	53.5	215-223 bp	1.0	43.7
chlN-psaF_p5	GAAAAGATCGGCTCTCAGCT	CTGAATAGTAATGAAAAACTATGAGT	50.0	26.9	57.7	53.7	213-221 bp	1.0	43.7