Hotspot

rps14-petG

LSC · 57705-57751 · 47 bp

Back to Ecklonia

Pi 0.0284

MarkerSeek score 64.60

Primer available yes

Label rps14-petG

Feature Metadata

label_name: rps14-petG
feature_type: igs
region: LSC
length_bp: 47
variable_sites: 2
pi: 0.0284
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-petG_p1	AATTGCTTACTGCCTGGTGT	CGTCGCGGAAATAAACTTGG	45.0	50.0	57.7	57.8	113 bp	1.0	77.8
rps14-petG_p2	TGCTTACTGCCTGGTGTAAG	CGTCGCGGAAATAAACTTGG	50.0	50.0	57.5	57.8	110 bp	1.0	76.4
rps14-petG_p3	ACTGCCTGGTGTAAGAAAGG	CGTCGCGGAAATAAACTTGG	50.0	50.0	57.4	57.8	105 bp	1.0	75.7
rps14-petG_p4	GGTCGTAGTCGAGGGGTTTA	CGTCGCGGAAATAAACTTGG	55.0	50.0	58.5	57.8	179 bp	1.0	75.4
rps14-petG_p5	GCTTACTGCCTGGTGTAAGA	CGTCGCGGAAATAAACTTGG	50.0	50.0	57.2	57.8	109 bp	1.0	74.1